h8 human cervical epithelial cell line  (ATCC)

Journal: RNA Biology

Article Title: Circular RNA Circ_0002762 promotes cell migration and invasion in cervical squamous cell carcinoma via activating RelA/nuclear factor kappa B (Nf-kB) signalling pathway

doi: 10.1080/15476286.2025.2478539

Figure Lengend Snippet: Circ_0002762 is up-regulated in CSCC cells (A) GSE102686 was analysed to screen for circRNAs aberrantly expressed in CSCC, leading to the identification of circ_0081672 and circ_002762 as candidate circRnas. (B) Expression profiles of circ_0002762 and circ_0081672 in CSCC tissues and paired para-cancerous cervical tissues were obtained from GSE102686. (C) Rt-qPCR was performed to examine expression levels of circ_0002762 and circ_0081672 in the three CSCC cell lines and cervical epithelial cell line (H8), revealing that circ_0002762 is highly expressed in CSCC cells. (D) Schematic diagram was presented to illustrate the splicing pattern of circ_0002762 (312nt). (E) Following ActD treatment, Rt-qPCR analysis showed that the mRNA stability of circ_0002762 is stronger than that of CDK17 mRNA. (F) RNA extractions were treated with RNase R and remaining levels were quantified with Rt-qPCR, indicating a significant level of circ_0002762. ** p < 0.01; N.S.: no significance.

Article Snippet: Cervical epithelial cell line H8 and three cervical cancer cell lines including HeLa, CaSki and C33A were procured from the American Type Culture Collection (ATCC).

Techniques: Expressing, Quantitative RT-PCR

Journal: Balkan Medical Journal

Article Title: ELK4 Promotes Cell Cycle Progression and Stem Cell-like Characteristics in HPV-associated Cervical Cancer by Regulating the FBXO22/PTEN Axis

doi: 10.4274/balkanmedj.galenos.2023.2023-4-66

Figure Lengend Snippet: ELK4 is highly expressed in HPV+ CC patients. (a) The mRNA expression of ELK4 in cervical tissues was compared with cervix cancer tissues by qRT-PCR. (b) Western blot assay showed the protein expression of ELK4 in cervical tissues and cervix cancer tissues. β-actin was normalized control. (c) ELK4 protein levels in human cervical epithelial immortalized cell line H8 and Cervix cancer cell lines (HeLa, CaSki, and SiHa) were detected by western blot. β-actin was normalized. The data are expressed as the mean ± SD. ***p < 0.001 vs normal tissues or cell line H8. SD, Standard deviation

Article Snippet: The human cervical epithelial immortalized cell line H8 (PCS-480-011) and cervical carcinoma cell lines such as HeLa (HPV18, CRM-CCL-2), CaSki (HPV16, CRM-CRL-1550), and SiHa (HPV − , HTB-35) (USA) were obtained from the American Type Culture Collection.

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Control, Standard Deviation